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The file was probably the shortcut for HTC_Guardian in the Windows\Startup folder.

I've read a few posts that in order to stop HTC_Guardian on the touch you need to find or make a dummy .exe that just exits, rename that .exe to HTC_Guardian.exe and copy it on top of the existing HTC_Guardian.exe. You can't rename or delete the one that's there because it's in ROM. But you can put something in place of it that does nothing.

Note: I may have the name slightly wrong.